Topographic single cell sequencing tscs
WebOct 21, 2024 · Optimized SCS-based strategies such as transposase-accessible chromatin with sequencing (ATAC-seq), single-nucleus RNA sequencing (snRNA-seq), single-cell … WebTo overcome these challenges, we developed Topographic Single Cell Sequencing (TSCS), which combines laser-catapulting with single cell DNA sequencing to measure genomic copy number profiles from single tumor cells while preserving their spatial context. We applied TSCS to sequence 1,293 single cells from 10 synchronous DCIS patients.
Topographic single cell sequencing tscs
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WebApr 1, 2024 · By developing a spatially resolved scDNA-seq method called Topographic Single Cell Sequencing (TSCS), genomic copy number aberrations (CNAs) were linked to spatial information in 10 cases of synchronous DCIS-IDC tissues, which showed that multiple clones co-migrated from the ducts into the invasive regions (Casasent et al., 2024). WebAug 2, 2024 · This method, termed topographic single-cell sequencing (TSCS), was used to examine genomic lineages in ductal carcinoma in situ and invasive ductal carcinoma …
WebJan 18, 2024 · Using a similar strategy, topographic single cell sequencing (TSCS) is designed to capture the genomic copy number profile of single tumour cells spatially. 17 By utilising TSCS in breast cancer samples, the results show a direct genomic lineage of breast cancer cell progression. Interestingly, the authors observe that most mutations and copy ...
WebCasasent et al. developed a method called Topographic Single Cell Sequencing (TSCS), which utilizes LCM followed by single-cell DNA-sequencing, to measure genomic copy number profiles of single tumor cells while preserving their spatial context in breast cancer patients with both ductal carcinoma in situ (DCIS) and invasive ductal carcinoma (IDC) . WebAug 2, 2024 · This method, termed topographic single-cell sequencing (TSCS), was used to examine genomic lineages in ductal carcinoma in situ and invasive ductal carcinoma breast cancer 87, demonstrating the ...
WebJan 3, 2024 · Findings were published in the Jan. 4 on-line issue of Cell. The discovery was made possible by the researchers’ development of a new analytical tool called …
WebJan 4, 2024 · The discovery was made possible by the researchers’ development of a new analytical tool called topographic single cell sequencing (TSCS). “While DCIS is the most … thz science and technologyWebTopographic single cell sequencing (TSCS) TSCS reveals accurate spatial information for each cell, preserving their spatial context in tissue sections. This enables researchers to spatially map the specific characteristics of individual ... Single-cell sequencing enabled the characterization of multiple sub-populations of cells in specific ... thz sensing reviewWebJan 23, 2024 · A variety of singlecell genome sequencing technologies have become powerful tools for detecting cellular heterogeneity, such as single-cell genomic sequencing (SIC-seq), single-cell combinatorial marker sequencing (SCI-seq), and topographic single cell sequencing (TSCS) (Navin et_al, 2011; Vitak et_al, 2024; Casasent et_al, 2024). thz sarWebThe advent of new sequencing technologies, the miniaturisation of reaction volumes, and the associated reagent usage have considerably reduced the costs of single-cell sequencing . These robust advances and cost savings in tandem have enabled the growing footprint and application of single-cell sequencing in disease biology and basic research. thz smart fftWebJul 1, 2024 · To overcome these challenges, we developed Topographic Single Cell Sequencing (TSCS) to measure genomic copy number profiles of single tumor cells while preserving their spatial context in tissue sections. We applied TSCS to 1,293 single cells from 10 synchronous patients with both DCIS and IDC regions in addition to exome … the law of independentWebJan 4, 2024 · This hinders single-cell sequencing, because it contaminates samples with microbial species that distract the polymerases used in whole-genome amplification. … thz si harmonicsWebFeb 18, 2024 · TSCS: Topographic single-cell sequencing. WGA: Whole genome amplification. References. Siegel RL, Miller KD, Jemal A (2024) Cancer statistics, 2024. CA Cancer J Clin 69(1):7–34. Article PubMed Google Scholar Bocci F et al (2024) Toward understanding cancer stem cell heterogeneity in the tumor microenvironment. … the law of independent assortment definition