Lifeact-tdtomato
Web11. maj 2024. · To test whether Spizellomyces zoospores crawl using α-motility, we expressed a LifeAct-tdTomato fusion. LifeAct is a 17 amino acid peptide that binds … Web25. nov 2024. · 在所有光谱型中，亮度最强的荧光蛋白是串联形式的二聚体Tomato（dimeric Tomato, dTomato）。. 它是一种原始的水果蛋白的橙色衍生物。. dTomato来源于一种被命名为dimer2的中间体，这种中间体是在四聚体DsRed蛋白崩解过程中产生的。. 这种变体包含GFP的N末端和C末端的 ...
Lifeact-tdtomato
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WebLifeAct — это специфический краситель, который связывается и окрашивает филаменты актина (F-актин) в эукариотических клетках. Он представляет собой … Web30. sep 2024. · To test whether Spizellomyces zoospores crawl using α-motility, we expressed a LifeAct-tdTomato fusion. LifeAct is a 17 amino acid peptide that binds specifically to polymerized actin in a wide variety of cell types, such as actin patches and cables in yeast and actin-filled protrusions of crawling animal cells (27, 28).
Web07. jan 2024. · The Lifeact sequence was initially reported in Riedl, J. et al. Lifeact: a versatile marker to visualize F-actin. Nat Methods 5, 605 607 (2008). Please cite the following article as follows: Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Web28. apr 2024. · COS-7 cell transiently transfected with Tomm20-mEmerald and Calreticulin-tdTomato. Tomm20 labels the outer membrane of mitochondria, Calreticulin is a protein of the endoplasmic reticulum (ER) where proteins are synthesized. ... U2OS cells expressing Lifeact-tdTomato and stained with MitoTracker Green. Top row: Single-camera …
Web09. jan 2015. · 3T3-L1 adipocytes expressing Lifeact-tdTomato were serum starved for 2 h and imaged using TIRFM. Insulin was added at zero time point and TIRF images were taken over 30 min with an interval of 15 s. WebLifeAct (N terminal on insert) Cloning Information Cloning method Ligation Independent Cloning 5′ sequencing primer tttatgctccaagcggagac 3′ sequencing primer …
WebLentiviral particles for fluorescent whole-cell labeling. Cells transduced with these premade, super-high titer particles will express a bright fluorescent protein in one of four colors: red …
WebA membrane-localized tdTomato gene (Myr-tdTomato) was linked to a nuclear-localized EGFP gene (H2B-EGFP) with the 2A sequence. The tdTomato is a red fluorescent protein and contains a tandem repeat of two copies of dTomato (Shaner et al. 2004). The TAG expression and 2A function have been assessed both in developmental stages and in … halakkiWeb20. apr 2024. · Lifeact, a 17 amino-acid peptide, when fused to tdTomato, stains F-actin structures without interfering actin dynamics in vitro or in vivo . Upon AMPK activation, increased F-actin remodeling was observed in WT cells as shown by increased Lifeact-tdTomato fluorescence under TIRFM ( Figures 5A, B and Supplementary Figure 5B and … hala tenisowaWebPlasmid pLenti-Lifeact-tdTomato from Dr. Weiping Han's lab contains the insert Lifeact and is published in Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951. This … halakoeiWebover-expression. In addition, it also shows colocalization between Lifeact-tdTomato and phalloidin staining in axons from adult retina explants and illustrates the maintenance of phalloidin staining after laser cut in the tip of the axon with the over-expression of DCLK2 and DCX-SP, compare to PLAP and DCX-270. halal store pullmanWeb30. nov 2014. · In the following study, we used targeted probes against F-actin (Lifeact-mTurquoise2), microtubules (EB1-venus), the nucleus (tdTomato-NLS) in comparison to … halal reisenWebPlasmid tdTomato-Lifeact-7 from Dr. Michael Davidson's lab is published in Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5311-5. doi: 10.1073/pnas.1119262109. Epub 2012 Mar … halal supermarket milton keynesWebBrand: AMICUS. Model: 20160768. LACTOACT TABLETS. Product description: LactoAct (lactose 3300 FCC) breaks down lactose entered food, and thus allows the consumption … halali noten