WebThe hydrophobic photoaffinity label 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine was used to label Sendai virus proteins during fusion with cardiolipin and phosphatidylserine liposomes. Preferential labeling of the viral fusion protein during the initial stages of fusion demonstrated that this protein interacts with the hydrophobic core of the target … WebPreferential labeling of the viral fusion protein during the initial stages of fusion demonstrated that this protein interacts with the hydrophobic core of the target membrane as an initiating event of virus-liposome fusion. Labeling showed time, temperature, and pH dependence consistent with earlier fluorescent measurements of fusion kinetics.

Current advances of carbene-mediated photoaffinity …

Web1 nov. 1990 · Determination by photoaffinity labelling of the hydrophobic part of the binding site for acyl-CoA esters on acyl-CoA-binding protein from bovine liver DOI: … Webthe hydrophobic F1 NH2terminus, consisting of about 20 aminoacids, into the target membrane(1, 3, 5, 6). Hydrophobic protein-lipid interactions (7-10) and some proteins that cause membrane fusion (11, 12) have been investigated by using photoaffinity labels. Such studies typically involve labeling ofboth protein and lipid after an section 8 raleigh nc apartments

Photoaffinity labelling of the Human GM2-activator protein

Web15 aug. 2013 · labeling reagents relating to their (1) elevated chemical stability and relatively easier synthesis as compared to azides, diazirines, and diazo compounds; (2) … Web13 feb. 2024 · Photoaffinity labeling confirms the HAP_R01 binding pose at the dimer-dimer interface on capsid and suggests a new mechanism of HAP-induced mis-assembly. Based on the common features in crystal... Web英文名称. photoaffinity labeling. 定 义. 应用化学标记试剂R-P的亲和标记法。. 其中R能特异、可逆地与拟标记分子的活性部位结合，P是在黑暗中不起反应的基团，经光激活作用 … section 8 raleigh north carolina