WebA membrane-bound sialidase in pig liver microsomes was solubilized with a nonionic detergent, IGEPAL CA630, and purified to homogeneity by sequential chromatographies on SP-Toyopearl, Butyl-Toyopearl (1st), SuperQ-Toyopearl, Hydroxyapatite, Butyl-Toyopearl (2nd), GM1-Cellulofine affinity, and sialic acid-Cellulofine affinity columns. WebOct 29, 2024 · The data also showed that Toyopearl Phenyl-650M and Butyl‑650M resins bound the oligonucleotide stronger compared with the Toyopearl PPG-600M resin. Toyopearl Hexyl-650C resin had the …
Hydrophobic Interaction Chromatography
WebApr 1, 1984 · Butyl-Toyopearl 650, a butyl derivative of Toyopearl HW-65, was synthesized for use in hydrophobic chromatography. Water-soluble enzyme proteins … WebThe extracellular lipase from Fusarium sp. YM-30 was purified by a procedure involving ultrafiltration, ammonium sulfate precipitation, and DEAE-Toyopearl 650M, CM … fine dining events
TOYOPEARL Phenyl-650
WebProduct name : TOYOPEARL® Butyl-650M Bulk Media Product Number : 807477 Brand : Supelco 1.2 Relevant identified uses of the substance or mixture and uses advised against Identified uses : Laboratory chemicals, Synthesis of substances 1.3 Details of the supplier of the safety data sheet Company : Sigma-Aldrich Inc. 3050 SPRUCE ST ST. WebJul 1, 2024 · To obtain a homogeneous enzyme, the hydrolase was purified from the cell-free extract of Arthrobacter sp. K5 through five steps, including ammonium sulfate fractionation, ion-exchange chromatography on DEAE-Sephacel, and hydrophobic interaction chromatography on phenyl-Sepharose and butyl-Toyopearl . WebPhosphodiesterase I [EC 3.1.4.1] was purified from normal human urine in a highly purified state free from phosphodiesterase II, RNase, DNase I, DNase II, and phosphatase by column chromatographies of DEAE-Toyopearl, butyl-Toyopearl, Affi-Gel blue, and Sephadex G-150. The molecular weight of the enzyme was 1.9 x 10 (5) and the pH … fine dining essex