2024 Adipose tissue at single-cell resolution

Adipose tissue at single-cell resolution

Author: swkc

August undefined, 2024

WebApr 12, 2024 · An integrated single cell and spatial transcriptomic map of human white adipose tissue, Nat Commun. 2024 Mar 15;14(1):1438. Borges TJ, Abarzua P, Gassen RB, Kollar B, Lima-Filho M, Aoyama BT, et al. T cell-attracting CCL18 chemokine is a dominant rejection signal during limb transplantation. Cell Rep Med. 2024;3(3):100559. WebTwo clusters of mature adipocytes, identified through single nuclei RNA sequencing (snRNA-seq), were distinctive for proinflammatory/immune or metabolic gene expression patterns that became more transcriptionally diverse in adult animals.

Single cell analysis reveals immune cell–adipocyte crosstalk

WebTo identify which adipose tissue cell types are mainly responsible for ECM remodeling, ... Plasticity of epididymal adipose tissue in response to diet-induced obesity at single-nucleus resolution. Cell Metab., 33 (2024), pp. 437-453.e5. View PDF View article View in Scopus Google Scholar. 31. WebJun 21, 2024 · An in-depth elucidation of the cellular alterations in subcutaneous adipose tissues of lymphedema is essential for medical development. We performed single-cell RNA sequencing of 70,209 cells of the stromal vascular fraction of adipose tissues from lymphedema patients and healthy donors. nickmercs going to youtube gaming

Adipose tissue at single-cell resolution: Cell Metabolism

WebJul 18, 2024 · Here we examine an established population of human adipose-derived stem cells (ASCs) using a novel, microfluidic-based method for high-throughput transcriptional interrogation, coupled with advanced bioinformatic analysis, to evaluate the dynamics of single cell gene expression among primary, passage 0, and passage 1 stem cells. WebAging usually comes associated with increased visceral fat accumulation, reaching even an obesity state, and favoring its associated comorbidities. One of the processes involved in … WebMay 13, 2024 · Adipose tissue is a multicellular tissue with depot-dependent functions including safe energy storage and release, immune defense, thermogenesis, and organ padding. Emont et al. zoom in on white adipose tissue at a single-cell resolution and provide an extensive resource for future insights in how to target obesity. 1 nickmercs gears of war gameplay

Multidimensional Single-Nuclei RNA-Seq Reconstruction of Adipose Tissue ...

Adipose Tissue - an overview ScienceDirect Topics

Webadipose tissue, or fatty tissue, connective tissue consisting mainly of fat cells (adipose cells, or adipocytes), specialized to synthesize and contain large globules of fat, within a structural network of fibres. It is found … WebOct 10, 2024 · Single-cell atlas and mesenchymal stem cell state characterization (A) Single-cell atlas of 204,883 cells across three tissues and four intervention groups. The tSNE plot is colored by cell type (warm colors, non-immune cell types; cold colors, immune cell types). (B and C) Single-cell atlas colored by tissue (B) and intervention group (C). nickmercs height ftWebMay 13, 2024 · Adipose tissue is a multicellular tissue with depot-dependent functions including safe energy storage and release, immune defense, thermogenesis, and organ … novotel perth contact details

"WebHistorically, the study of adipose tissue has been facilitated by classical techniques such as bioimaging, two-dimensional (2D) cell culture, flow cytometry, and mouse genetics. However, the increasing understanding of tissue heterogeneity and the need for more physiological in vitro models have also highlighted their limitations. " - Adipose tissue at single-cell resolution

Adipose tissue at single-cell resolution

Single-cell RNA sequencing of subcutaneous adipose tissues …

WebThe emergence of single-cell transcriptomics has rapidly transformed our understanding of the diverse array of cell types and cell states residing in adipose tissues and has … WebJul 5, 2024 · Although the approach does not guarantee measurements at single-cell resolution, it does not require single-cell isolation and may be amendable for …

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WebApr 27, 2024 · Human adipose tissue was isolated from healthy patients (patient information in Supplementary Table 1 ), dissociated into single-cell suspensions, sorted … WebApr 14, 2024 · Abstract. Adipose tissue in the mammary gland undergoes dramatic remodeling during obesity and is involved in numerous metabolic diseases. However, how obesity-driven remodeling in adipose tissue regulates anti-tumor immunity remains unclear. Here we provide the detailed cellular atlases of mouse mammary fat pad and …

WebMay 13, 2024 · Adipose tissue is a multicellular tissue with depot-dependent functions including safe energy storage and release, immune defense, thermogenesis, and organ padding. Emont et al. zoom in on white adipose tissue at a single-cell resolution and provide an extensive resource for future insights in how to target obesity. WebJun 6, 2024 · The ultimate goal of this protocol is to determine all adipose tissue subtypes under various thermogenic stimuli at a single-cell resolution. In contrast to other tissues and cell types, determining cellular subtypes of adipose tissue is challenging due to the fragility of lipid-filled adipocytes.

WebMar 23, 2024 · Abstract. Technologies allowing studies at single-cell resolution have provided important insights into how different cell populations contribute to tissue function. Application of these methods to white adipose tissue (WAT) has revealed how various metabolic aspects of this organ, such as insulin response, inflammation and tissue … WebFeb 27, 2024 · Highlights Multidimensional transcriptome analysis at single-nucleus resolution recovers nuclei of cell types in adipose tissue Adaptative thermogenic response results in 3 distinct mature adipose cell types Single-nuclei transcriptomic-based secretome analysis reveals adipose cell-type-specific genes

WebFeb 15, 2024 · Adipose tissue is highly heterogeneous and plastic. Recent advances in single-cell/nucleus RNA sequencing technology have helped to study the cellular composition and dynamics of adipose tissue. In this protocol, I outline a typical workflow of analyzing single-cell/nucleus transcriptome data.

WebApr 3, 2024 · Fat grafting is one example of an autologous technique where autologous adipose tissue (isolated from a donor site via liposuction) is injected into the breast. However, it lacks structural support and vasculature, which results overtime in a stress-induced volume loss of 20%–70% due to the applied forces (Visscher et al., 2024), … novotel perth langleyWebMar 7, 2024 · Adipose tissue exhibits remarkable plasticity with capacity to change in size and cellular composition under physiological and pathophysiological conditions. The … nickmercs house floridaWebAdipose tissues display a remarkable ability to adapt to the dietary status. Here, we have applied single-nucleus RNA-seq to map the plasticity of mouse epididymal white … novotel paris centre eiffel towerWebOct 28, 2024 · Adipose tissue is usually classified on the basis of its function as white, brown or beige (brite) 1. It is an important regulator of systemic metabolism, as shown by … novotel perth langley parkingWebApr 12, 2024 · Adipose tissue is a specialized connective tissue consisting of lipid-rich cells called adipocytes. As it comprises about 20-25% of total body weight in healthy individuals, the main function of adipose tissue is to store energy in the form of lipids (fat). nickmercs house tourWebMar 7, 2024 · Single-cell technologies: Promising path to resolving adipose tissue function and heterogeneity The advent of NGS-based single-cell technologies has allowed for … novotel perth langley australiaWebMar 7, 2024 · Adipose tissues are loose connective tissues with a high number of adipocytes. These specialized cells store metabolic energy in the form of large cytoplasmic lipid droplets and at the same time regulate several aspects of organismal physiology … novotel perth murray